Additional Selective and Differential Cell Culture Media
NEOGEN Tetrathionate Broth Base, 10 kg
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Bismuth Sulphite Agar, 10 kg
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar
NEOGEN Tetrathionate Broth Base, 500 g
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Malt Extract Agar, 5 kg
Malt Extract Agar is used for the cultivation of fungi and is not intended for use in the diagnosis of disease or other conditions in humans. An acidic medium which will support the growth of most yeasts and molds whilst inhibiting most bacteria. It was first described by Thom and Church in 1926 in a study of Aspergillus spp. claiming the high carbohydrate content ensured rapid growth. Selectivity can be increased by further lowering the pH with the addition, after sterilization, of Lactic Acid (X037). It should be noted that excess heating of this medium together with its low pH can easily result in hydrolysis of the agar gel producing soft plates. Formula:Malt Extract - 30.0 g/LPeptone - 5.0 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/malt-extract-agar
NEOGEN Peptone Water, 5 kg
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies. The medium has a high level of tryptone making it suitable for use in the indole test.For more information visit https://www.neogen.com/solutions/Microbiology/peptone-water
NEOGEN Peptone Water, 500 g
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies. The medium has a high level of tryptone making it suitable for use in the indole test.For more information visit https://www.neogen.com/solutions/Microbiology/peptone-water
NEOGEN Campy Cefex Agar, 10 kg
Campy Cefex Agar is used for the selective isolation of Campylobacter spp in a laboratory setting. Campy Cefex Agar is not intended for use in the diagnosis of disease or other conditions in humans.Poultry is a primary reservoir of Campylobacter spp. and may cause contamination of more than 80% of chicken carcasses. Campy Cefex Agar is described by Stern et al. This medium was formulated to provide selective isolation of cephalothin-resistant Campylobacter species such as C. jejuni and C. coli.For more information visit https://www.neogen.com/solutions/Microbiology/campy-cefex-agar
NEOGEN Campylobacter Enrichment Broth (Bolton Broth), 5 kg
Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans. Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton Broth (2X Concentration) is described by the USDA.For more information visit https://www.neogen.com/solutions/Microbiology/campylobacter-enrichment-broth-bolton
NEOGEN Sabouraud Dextrose Agar with Lecithin & Tween, 500 g
Sabouraud Dextrose Agar (with Lecithin & Tween) is used for the isolation of fungi from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Sabouraud Dextrose Agar (with Lecithin & Tween) is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. The addition of Lecithin and Tween to Sabouraud Dextrose Agar is used to neutralize antiseptics and disinfectants for environmental monitoring and other applications. Complete neutralization of disinfectants is important. Disinfectant carryover can cause false no-growth test results.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-with-lecithin-and-tween
NEOGEN XLT4 Agar, 500 g
XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp and is not intended for use in the diagnosis of disease or other conditions in humans. XLT4 Agar is a selective differential isolation medium for the specific detection of Salmonella spp. from environmental and food samples. Due to its highly selective nature, XLT4 Agar is particularly effective when used with samples where overgrowth of contaminating flora is expected, for example, fecally contaminated agricultural samples. Developed to perform as per Miller & Tate in 1990, this medium was found to improve the recovery of non-typhi Salmonella from chicken and farm environmental samples. Dusch and Altwegg further established the application of XLT4 Agar to salmonellae detection in clinical samples, with the notable exceptions of Salmonella typhi and Salmonella paratyphi.For more information visit https://www.neogen.com/solutions/Microbiology/xlt4-agar
NEOGEN Eosin Methylene Blue (EMB) Agar Levine, 500 g
Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.For more information visit https://www.neogen.com/solutions/Microbiology/eosin-methylene-blue-agar-levine
NEOGEN EC Medium with MUG, 500 g
EC Medium w/ MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium w/ MUG is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium w/ MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-ß-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-ß-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 ɥg/mL.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-with-mug
NEOGEN Rappaport Vassiliadis Salmonella Enrichment Broth, 500 g
Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopoeia for the enrichment and selective isolation of Salmonella spp.from non-sterile samples. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. enteritidis) are known to be sensitive to malachite green and as such may fail to grow.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-salmonella-enrichment-broth
NEOGEN Cetrimide Agar
Cetrimide Agar is used in the isolation and identification of Pseudomonas aeruginosa and is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements. A medium recommended by the Harmonized USP/EP/JP for the isolation and identification of Pseudomonas aeruginosa, in non- sterile pharmaceutical samples. Conforms to USP/EP/JP performance specification. Gelatin is a source of nitrogen while glycerol acts as a carbon source. Cetrimide is a quaternary ammonium compound that inhibits the growth of a wide range of Gram-positive and some Gram-negative micro-organisms. Magnesium chloride and Dipotassium sulphate improve the production of pyoverdin and pyocyanin pigments that combine to give Pseudomonas aeruginosa characteristic green colonies.For more information visit https://www.neogen.com/solutions/Microbiology/cetrimide-agar
NEOGEN Rappaport Vassiliadis R10 Broth, 500 g
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-r10-broth